This exclusive fluorophore features the highest quantum yield of any calcium indicator on the market, delivering the greatest signal window available in a calcium assay kit. The substantial increase in signal amplitude helps to rekindle low signal screens; including endogenous, primary or stem cell targets. Additionally, the novel dye is unaffected by organic anion exchange proteins, enabling measurement with minimal to no probenecid present.

The quench-free (QF) formulation is ideal for quench sensitive targets or assays requiring multiplexing of calcium-flux and luminescent assays within one well.

Key Assay Features and Benefits

• Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts
• Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput screens
• Universal mix-and-read protocol accelerates assay workflow and increases throughput
• Superior signal-to-noise ratio facilitates confirmation of endogenous or transiently transfected receptor activity during assay development
• Pre-optimized and validated protocols for the FLIPR^® Tetra and FlexStation^® Systems ensure you can navigate both routine and unconventional cell lines and targets

Available Configurations: