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25% off the list price. Promotion is available in the United States only when buying directly from Molecular Devices. Purchase order must reference promotion code 25AssayKits17. The offer is to purchase any of the following: EarlyTox Cell Viability Assay Kits, SpectraMax Glo Steady-Luc Reporter Assay Kit, or SpectraMax Quant dsDNA Assay Kit. Valid May 25, 2017 through August 31, 2017. The offer does not include any other options or accessories. This promotion cannot be combined with any other promotions or discounts. No substitutions or swap outs. The discount is not convertible to cash. All prices exclude delivery charges and local taxes, which are the responsibility of the customer. Purchase orders must be received by August 31, 2017. Promotion is not valid for standing orders. The purchase is subject to Molecular Devices standard terms and conditions of sale. All products are for research use only. Void where prohibited. Product Purchase Terms – US.
EarlyTox Caspase-3/7 NucView 488 Assay Kit
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This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. NucView 488 Caspase-3 substrate (formulated in DMSO or phosphate-buffered saline (PBS), for EarlyTox Caspase-3/7-D NucView 488 Assay Kit and EarlyTox Caspase-3/7 NucView 488 Assay Kit, respectively) is used to detect caspase-3/7 activity within intact cells without interfering with caspase activity. The substrate consists of a fluorogenic DNA dye that is coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the substrate permeates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus and stains DNA with bright green fluorescence, with excitation at 500 nm and emission at 530 nm. NucView 488 staining is formaldehyde-fixable and compatible with subsequent immunostaining. The assay kit includes an optional masking reagent to reduce background fluorescence when necessary. The PBS formulation is recommended for cells that are sensitive to DMSO.
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