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FLIPR Calcium 6-QF Assay Kit

FLIPR Calcium 6-QF Assay Kit

  • $671.00




This exclusive fluorophore features the highest quantum yield of any calcium indicator on the market, delivering the greatest signal window available in a calcium assay kit. The substantial increase in signal amplitude helps to rekindle low signal screens; including endogenous, primary or stem cell targets. Additionally, the novel dye is unaffected by organic anion exchange proteins, enabling measurement with minimal to no probenecid present.

The quench-free (QF) formulation is ideal for quench sensitive targets or assays requiring multiplexing of calcium-flux and luminescent assays within one well.

Key Assay Features and Benefits

  • Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts
  • Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput screens
  • Universal mix-and-read protocol accelerates assay workflow and increases throughput
  • Superior signal-to-noise ratio facilitates confirmation of endogenous or transiently transfected receptor activity during assay development
  • Pre-optimized and validated protocols for the FLIPR® Tetra and FlexStation® Systems ensure you can navigate both routine and unconventional cell lines and targets

 

 

Available Configurations:

 Configuration Description Part Number
Explorer Kit
  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)
  • (10) vials of component C

*  Each reagent vial (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

(R8192)
Bulk Kit
  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)
  • (10) vials of component C
*  Each reagent vial (Component A, C) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.
(R8193)
Express Kit
  • (2) vials of component A*
  • (1) bottle of dilution buffer (Component B)
  • (2) vials of component C
* Each reagent vial (Component A, C) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.
    (R8196)

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