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EarlyTox Caspase-3/7 R110 Assay Kit

*Terms and Conditions
25% off the list price. Promotion is available in the United States only when buying directly from Molecular Devices. Purchase order must reference promotion code 25AssayKits17. The offer is to purchase any of the following: EarlyTox Cell Viability Assay Kits, SpectraMax Glo Steady-Luc Reporter Assay Kit, or SpectraMax Quant dsDNA Assay Kit. Valid May 25, 2017 through August 31, 2017. The offer does not include any other options or accessories. This promotion cannot be combined with any other promotions or discounts. No substitutions or swap outs. The discount is not convertible to cash. All prices exclude delivery charges and local taxes, which are the responsibility of the customer. Purchase orders must be received by August 31, 2017. Promotion is not valid for standing orders. The purchase is subject to Molecular Devices standard terms and conditions of sale. All products are for research use only. Void where prohibited. Product Purchase Terms – US.

EarlyTox Caspase-3/7 R110 Assay Kit

  • $345.00

25% OFF* Use Code 25AssayKits17 at Check Out





This kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically designed for microplate readers. The fluorogenic substrate (Ac-DEVD)2-R110 contains two DEVD consensus target sequence and is completely hydrolyzed in cell lysate by the enzymes. Hydrolysis of both DEVD peptides releases the green fluorescent dye R110, resulting in a substantial fluorescence increase, with excitation at 490 nm and emission at 520 nm. The assay kit includes the competitive caspase-3/7 inhibitor Ac-DEVD-CHO for use as a negative control. R110 is also provided in the kit for generating a standard curve, which can be used for quantifying caspase-3 activity.  

     

     

    Available Configurations:

     Configuration Description Part Number

    Explorer Kit

    • (1) vial of Rhodamine 110 (80 µM, 1 mL)
    • (2) vials of caspase-3 inhibitor (5 mM, 20 µL)
    • (2) vials of DEVD-R110 (2 mM, 500 µL)
    • (2) vials of lysis buffer (10 mL)

      Each kit contains enough material for two (2) 96-well or 384-well microplates.
    (R8346)
    Bulk Kit
    • (1) vial of Rhodamine 110 (80 µM, 1 mL)
    • (2) vials of caspase-3 inhibitor (5 mM, 100 µL)
    • (1) vial of DEVD-R110 (2 mM, 5 mL)
    • (1) vial of lysis buffer (100 mL)

      Each kit contains enough material for ten (10) 96-well or 384-well microplates.
    (R8347)

     

     


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