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IMAP Assay Kit

IMAP Assay Kit

  • $847.00


For more information on assay kit and instrument compatibility, please click here.



Please note: All purchases should be limited to United States.

For complete Terms and Conditions, please click here.


Based on the specific, high-affinity interaction of phospho groups with trivalent metal-containing nanoparticles (beads), IMAP is a generic, non-antibody-based platform to assess kinase, phosphatase, and phosphodiesterase activity. An enzyme reaction is performed using fluorescently labeled substrate. Addition of the IMAP Binding System stops the enzyme reaction and initiates binding of the beads to phosphorylated substrates. Binding of the substrate to the beads, which correlates to enzyme activity, can be detected using either FP or TR-FRET as a readout.

Benefits of IMAP Assays

  • IMAP provides a complete assay system for screening kinases, phosphatases, and phosphodiesterases
  • Because IMAP assays are not antibody-based, they are generic and can be used for any kinase, phosphatase, or phosphodiesterase
  • Robust fluorescence signal gives reliable results with good Z factors
  • IMAP assays are homogeneous and amenable to miniaturization for greater cost savings
  • IMAP assays are available in both FP and TR-FRET detection modes to meet users' screening needs

Available Configurations:

 Configuration Part Number

IMAP® EVALUATION DEMO KIT
For kinase, phosphatase and Phosphodiesterase fluorescent polarization or TR-FRET assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, labeld calibrators/substrates, TR-FRET Tb-donor to run a calibration curve for 800 data points in a standard 384 well format.

R8166

IMAP® FP EVALUATION KIT
For kinase and phosphatase fluorescent polarization assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, sufficient to generate 800 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.

R8155

IMAP® TR-FRET EVALUATION KIT
For kinase and phosphatase TR-FRET assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, TR-FRET Tb-donor, sufficient to generate 800 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.

R8161

IMAP® PDE FP EVALUATION KIT
For Phosphodiesterase (PDE) fluorescent polarization assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, labeld cAMP and cGMP substrates, sufficient to generate 800 data points in a standard 384 well format

R8175

IMAP® PDE TR-FRET EVALUATION KIT
For Phosphodiesterase (PDE) TR-FRET assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, labeld cAMP and cGMP substrates, TR-FRET Tb-donor, sufficient to generate 800 data points in a standard 384 well format

R8176

IMAP® FP SCREENING EXPRESS KIT
Fluorescence Polarization Screening Express Kit with Progressive Binding System. Consists of proprietary IMAP Binding Reagent and Binding Buffers, sufficient to generate 8,000 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.

R8127

IMAP® TR-FRET SCREENING EXPRESS KIT
TR-FRET Screening Express Kit with Progressive Binding System. Consists of proprietary IMAP Binding Reagent and Binding Buffers, TR-FRET Tb-donor, sufficient to generate 8,000 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.

R8160


IMAP Assay Components

Configuration Part Number

IMAP TR-FRET DONOR 8K dp
Tb-donor for IMAP TR-FRET assay, sufficient amount for screening and generate 8,000 data points in a standard 384 well format.

R7470

IMAP TR-FRET DONOR 50K dp
Tb-donor for IMAP TR-FRET assay, sufficient amount for high throughput screening and generate 50,000 data points in a standard 384 well format.

R7471

IMAP PDE cAMP SUBSTRATE 20 nmoles
Fluorescence labeled cAMP substrate 20 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 8,000 data points based on 100 nM in reaction. Replaced R7091

R7505
IMAP PDE cAMP SUBSTRATE 120 nmoles
Fluorescence labeled cAMP substrate 120 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 50,000 data points based on 100 nM in reaction.
R7506
IMAP PDE cGMP SUBSTRATE 20 nmoles
Fluorescence labeled cGMP substrate 20 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 8,000 data points based on 100 nM in reaction. Replaced R7090
R7507
IMAP PDE cGMP SUBSTRATE 120 nmoles
Fluorescence labeled cGMP substrate 120 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 50,000 data points based on 100 nM in reaction.
R7508
FAM-CROSSTIDE 8K dp
Fluorescence labeled peptide 5FAM-GRPRTSSFAEG-COOH, substrate for Akt1, Akt2, Akt3, MSK1, MSK2, SGK1, Plk3, sufficient for 8,000 data points based on 100 nM in reaction
R7110
FAM-EGFR-DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide LVEPLTPSGEAPNQK-5FAM-COOH, substrate for JNK1, JNK2,JNK3, p38 (α,β,γ,δ isoforms), sufficient for 8,000 data points based on 100 nM in reaction
R7129
FAM-P34CDC2-DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-KVEKIGEGTYGVV-NH2, substrate for Src, Fyn, Lck, Yes, Hck, Rse, sufficient for 8,000 data points based on 100 nM in reaction
R7157
FAM-P34CDC2-DERIVED PEPTIDE 50K dp
Fluorescence labeled peptide 5FAM-KVEKIGEGTYGVV-NH2, substrate for Src, Fyn, Lck, Yes, Hck, Rse, sufficient for 50,000 data points based on 100 nM in reaction
R7172
FAM-S6 RIBOSOMAL PROTEIN-DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-AKRRRLSSLRA-COOH, substrate for ROCK-II,Rsk1,Rsk2,Rsk3,Tab1,sufficient for 8,000 data points based on 100 nM in reaction
R7184
FAM-S6 RIBOSOMAL PROTEIN-DERIVED PEPTIDE 50K dp
Fluorescence labeled peptide 5FAM-AKRRRLSSLRA-COOH, substrate for ROCK-II,Rsk1,Rsk2,Rsk3,Tab1,sufficient for 50,000 data points based on 100 nM in reaction
R7229
FAM-PKATIDE 8K dp
Fluorescence labeled peptide 5FAM-GRTGRRNSI-NH2, substrate for Aurora A, PKA, PKG,sufficient for 8,000 data points based on 100 nM in reaction
R7250
FAM-HISTONE H1-DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-GGGPATPKKAKKL-COOH, substrate for CDK1/Cyclin B, CDK2/Cyclin A, CDK2/CyclinE, CDK3/CyclinE, CDK5/p25, CDK5/p35,CDK6/Cyclin D3,sufficient for 8,000 data points based on 100 nM in reaction
R7252
FAM-IκBα-DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-GRHDSGLDSMK-NH2, substrate for IKKβ, IKKα,sufficient for 8,000 data points based on 100 nM in reaction
R7254
FAM-PKATIDE 50K dp
Fluorescence labeled peptide 5FAM-GRTGRRNSI-NH2, substrate for Aurora A, PKA, PKG,sufficient for 50,000 data points based on 100 nM in reaction
R7255
FAM-HISTONE H1-DERIVED PEPTIDE 50K dp
Fluorescence labeled peptide 5FAM-GGGPATPKKAKKL-COOH, substrate for CDK1/Cyclin B, CDK2/Cyclin A, CDK2/CyclinE, CDK3/CyclinE, CDK5/p25, CDK5/p35,CDK6/Cyclin D3,sufficient for 50,000 data points based on 100 nM in reaction
R7257
FAM-ERKTIDE 8K dp
Fluorescence labeled peptide IPTTPITTTYFFFK-5FAM-COOH, substrate for Erk1, Erk2, p38 (α,β,γ,δ isoforms),sufficient for 8,000 data points based on 100 nM in reaction
R7292
TAMRA-EGFR-DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide LVEPLTPSGEAPNQK-5TAMRA-NH2, substrate for JNK1, JNK2, JNK3, p38 (α,β,γ,δ isoforms),sufficient for 8,000 data points based on 100 nM in reaction
R7307
FAM-CK1TIDE 8K dp
Fluorescence labeled peptide 5FAM-HAAIGDDDDAYSITA-NH2, substrate for CK1, CK1δ,sufficient for 8,000 data points based on 100 nM in reaction
R7311
FAM-CK1TIDE 50K dp
Fluorescence labeled peptide 5FAM-HAAIGDDDDAYSITA-NH2, substrate for CK1, CK1δ,sufficient for 50,000 data points based on 100 nM in reaction
R7312
TAMRA-PKATIDE 8K dp
Fluorescence labeled peptide 5TAMRA-GRTGRRNSI-COOH, substrate for PKA, PKG,sufficient for 8,000 data points based on 100 nM in reaction
R7313
FAM-EGFR-DERIVED PHOSPHOPEPTIDE 8K dp
Fluorescence labeled phosphopeptide LVEPL-pT-PSGEAPNQ(K-5FAM)-COOH, substrate for assay calibration,sufficient for 8,000 data points based on 100 nM in reaction
R7319
TAMRA-CDK7TIDE 8K dp
Fluorescence labeled peptide 5TAMRA-YSPTSPSYSPTSPSYSPTSPS-COOH, substrate for CDK7,sufficient for 8,000 data points based on 100 nM in reaction
R7352
TAMRA-ROSTIDE 50K dp
Fluorescence labeled peptide 5TAMRA-KKKSPGEYVNIEFG-NH2, substrate for Ros, Met,sufficient for 50,000 data points based on 100 nM in reaction
R7383
FAM-P38TIDE 8K dp
Fluorescence labeled peptide 5FAM-IPTTPITTTYFFFK-NH2, substrate for Erk1, Erk2, p38 (α,β,γ,δ isoforms),sufficient for 8,000 data points based on 100 nM in reaction
R7434
FAM-CAM KIADR/SYNAPSIN CHIMERA PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-LKKLRRRSDANF-NH2, substrate for CaMKI,sufficient for 8,000 data points based on 100 nM in reaction
RP7005
FAM-PKCε PSEUDOSUBSTRATE DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-RKRQGSVRRRVH-OH, substrate for PKC,sufficient for 8,000 data points based on 100 nM in reaction
RP7030
FAM-PKCα PSEUDOSUBSTRATE DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-RFARKGSLRQKNV-COOH, substrate for PKCζ,sufficient for 8,000 data points based on 100 nM in reaction
RP7032
FAM-IP3R-DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-GRRESLTSFG-NH2, substrate for PKA, PKG,sufficient for 8,000 data points based on 100 nM in reaction
RP7035
FAM-ALTERNATE SYNTIDE2 8K dp
Fluorescence labeled peptide 5FAM-PLSRTLSVSSLPGL-NH2, substrate for c-TAK,sufficient for 8,000 data points based on 100 nM in reaction
RP7045
FAM-CREBTIDE 8K dp
Fluorescence labeled peptide 5FAM-GEILSRRPSYRK-NH2, substrate for MSK1,sufficient for 8,000 data points based on 100 nM in reaction
RP7046
FAM-CDK7TIDE 8K dp
Fluorescence labeled peptide 5FAM-YSPTSPSYSPTSPSYSPTSPS-OH, substrate for TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction
RP7116
FAM-DYRKTIDE 8K dp
Fluorescence labeled peptide 5FAM-EPPQSQEAFADLWK-NH2, substrate for TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction
RP7119
FAM-P53 DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-RRRFRPASPLRGPPK-OH, substrate for Dyrk1A, TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction
RP7120
FAM-MBP DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-ATGPLSPGPFGRR-OH, substrate for Erk2, TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction
RP7123
FAM-P44 DERIVED PEPTIDE (AA200-218) 8K dp
Fluorescence labeled peptide 5FAM-FLTEYVATRWYRAPEIMLN-NH2, substrate for MAPK, TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction
RP7131
FAM-EXTENDED PLM DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-GTFRSSIRRLSTRRR-OH, substrate for Nek2, IRAK4,sufficient for 8,000 data points based on 100 nM in reaction
RP7140
FAM-RS DOMAIN DERIVED PEPTIDE 8K dp
Fluorescence labeled peptide 5FAM-GRSRSRSRSR-OH, substrate for SPRK1, IRAK4,sufficient for 8,000 data points based on 100 nM in reaction
RP7153
FAM-PKCε PSEUDOSUBSTRATE DERIVED PEPTIDE 50K dp
Fluorescence labeled peptide 5FAM-ERMRPRKRQGSVRRRV-NH2, substrate for PKCγ, Pim1,sufficient for 50,000 data points based on 100 nM in reaction
RP7548
FAM-GENERIC SER/THR PROTEIN KINASES SUBSTRATE PEPTIDE 50K dp
Fluorescence labeled peptide 5FAM-KKRKSSLRRWSPLTPRQMSFDC-NH2, substrate for PKA, PKC, CAMK, MAPK, TKL, STE, CK1, CMGC,sufficient for 50,000 data points based on 100 nM in reaction
RP7609
FAM-CDK7TIDE 50K dp
Fluorescence labeled peptide 5FAM-YSPTSPSYSPTSPSYSPTSPS-OH, substrate for TKL, STE, CK1, CMGC,sufficient for 50,000 data points based on 100 nM in reaction
FAM-EXTENDED PLM DERIVED PEPTIDE 50K dp
Fluorescence labeled peptide 5FAM-GTFRSSIRRLSTRRR-OH, substrate for Nek2, IRAK4,sufficient for 50,000 data points based on 100 nM in reaction
RP7640

 

Additional discounted configurations available for customers performing several high-throughput screens in a year.  Please contact your local sales representative or call 1-800-635-5577 for more information.


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