IMAP Assay Kit

$872.00

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Based on the specific, high-affinity interaction of phospho groups with trivalent metal-containing nanoparticles (beads), IMAP is a generic, non-antibody-based platform to assess kinase, phosphatase, and phosphodiesterase activity. An enzyme reaction is performed using fluorescently labeled substrate. Addition of the IMAP Binding System stops the enzyme reaction and initiates binding of the beads to phosphorylated substrates. Binding of the substrate to the beads, which correlates to enzyme activity, can be detected using either FP or TR-FRET as a readout.

Benefits of IMAP Assays

IMAP provides a complete assay system for screening kinases, phosphatases, and phosphodiesterases
Because IMAP assays are not antibody-based, they are generic and can be used for any kinase, phosphatase, or phosphodiesterase
Robust fluorescence signal gives reliable results with good Z factors
IMAP assays are homogeneous and amenable to miniaturization for greater cost savings
IMAP assays are available in both FP and TR-FRET detection modes to meet users' screening needs

Available Configurations:

Configuration	Part Number
IMAP® EVALUATION DEMO KIT For kinase, phosphatase and Phosphodiesterase fluorescent polarization or TR-FRET assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, labeld calibrators/substrates, TR-FRET Tb-donor to run a calibration curve for 800 data points in a standard 384 well format.	R8166
IMAP® FP EVALUATION KIT For kinase and phosphatase fluorescent polarization assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, sufficient to generate 800 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.	R8155
IMAP® TR-FRET EVALUATION KIT For kinase and phosphatase TR-FRET assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, TR-FRET Tb-donor, sufficient to generate 800 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.	R8161
IMAP® PDE FP EVALUATION KIT For Phosphodiesterase (PDE) fluorescent polarization assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, labeld cAMP and cGMP substrates, sufficient to generate 800 data points in a standard 384 well format	R8175
IMAP® PDE TR-FRET EVALUATION KIT For Phosphodiesterase (PDE) TR-FRET assays. Consists of proprietary IMAP Binding Reagent and Binding Buffers, labeld cAMP and cGMP substrates, TR-FRET Tb-donor, sufficient to generate 800 data points in a standard 384 well format	R8176
IMAP® FP SCREENING EXPRESS KIT Fluorescence Polarization Screening Express Kit with Progressive Binding System. Consists of proprietary IMAP Binding Reagent and Binding Buffers, sufficient to generate 8,000 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.	R8127
IMAP® TR-FRET SCREENING EXPRESS KIT TR-FRET Screening Express Kit with Progressive Binding System. Consists of proprietary IMAP Binding Reagent and Binding Buffers, TR-FRET Tb-donor, sufficient to generate 8,000 data points in a standard 384 well format. Labeled substrates are available to purchase seperatly.	R8160

IMAP Assay Components

Configuration	Part Number
IMAP TR-FRET DONOR 8K dp Tb-donor for IMAP TR-FRET assay, sufficient amount for screening and generate 8,000 data points in a standard 384 well format.	R7470
IMAP TR-FRET DONOR 50K dp Tb-donor for IMAP TR-FRET assay, sufficient amount for high throughput screening and generate 50,000 data points in a standard 384 well format.	R7471
IMAP PDE cAMP SUBSTRATE 20 nmoles Fluorescence labeled cAMP substrate 20 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 8,000 data points based on 100 nM in reaction. Replaced R7091	R7505
IMAP PDE cAMP SUBSTRATE 120 nmoles Fluorescence labeled cAMP substrate 120 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 50,000 data points based on 100 nM in reaction.	R7506
IMAP PDE cGMP SUBSTRATE 20 nmoles Fluorescence labeled cGMP substrate 20 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 8,000 data points based on 100 nM in reaction. Replaced R7090	R7507
IMAP PDE cGMP SUBSTRATE 120 nmoles Fluorescence labeled cGMP substrate 120 nmoles for IMAP phosphodiesterase (PDE) assays, sufficient for 50,000 data points based on 100 nM in reaction.	R7508
FAM-CROSSTIDE 8K dp Fluorescence labeled peptide 5FAM-GRPRTSSFAEG-COOH, substrate for Akt1, Akt2, Akt3, MSK1, MSK2, SGK1, Plk3, sufficient for 8,000 data points based on 100 nM in reaction	R7110
FAM-EGFR-DERIVED PEPTIDE 8K dp Fluorescence labeled peptide LVEPLTPSGEAPNQK-5FAM-COOH, substrate for JNK1, JNK2,JNK3, p38 (α,β,γ,δ isoforms), sufficient for 8,000 data points based on 100 nM in reaction	R7129
FAM-P34CDC2-DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-KVEKIGEGTYGVV-NH2, substrate for Src, Fyn, Lck, Yes, Hck, Rse, sufficient for 8,000 data points based on 100 nM in reaction	R7157
FAM-P34CDC2-DERIVED PEPTIDE 50K dp Fluorescence labeled peptide 5FAM-KVEKIGEGTYGVV-NH2, substrate for Src, Fyn, Lck, Yes, Hck, Rse, sufficient for 50,000 data points based on 100 nM in reaction	R7172
FAM-S6 RIBOSOMAL PROTEIN-DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-AKRRRLSSLRA-COOH, substrate for ROCK-II,Rsk1,Rsk2,Rsk3,Tab1,sufficient for 8,000 data points based on 100 nM in reaction	R7184
FAM-S6 RIBOSOMAL PROTEIN-DERIVED PEPTIDE 50K dp Fluorescence labeled peptide 5FAM-AKRRRLSSLRA-COOH, substrate for ROCK-II,Rsk1,Rsk2,Rsk3,Tab1,sufficient for 50,000 data points based on 100 nM in reaction	R7229
FAM-PKATIDE 8K dp Fluorescence labeled peptide 5FAM-GRTGRRNSI-NH2, substrate for Aurora A, PKA, PKG,sufficient for 8,000 data points based on 100 nM in reaction	R7250
FAM-HISTONE H1-DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-GGGPATPKKAKKL-COOH, substrate for CDK1/Cyclin B, CDK2/Cyclin A, CDK2/CyclinE, CDK3/CyclinE, CDK5/p25, CDK5/p35,CDK6/Cyclin D3,sufficient for 8,000 data points based on 100 nM in reaction	R7252
FAM-IκBα-DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-GRHDSGLDSMK-NH2, substrate for IKKβ, IKKα,sufficient for 8,000 data points based on 100 nM in reaction	R7254
FAM-PKATIDE 50K dp Fluorescence labeled peptide 5FAM-GRTGRRNSI-NH2, substrate for Aurora A, PKA, PKG,sufficient for 50,000 data points based on 100 nM in reaction	R7255
FAM-HISTONE H1-DERIVED PEPTIDE 50K dp Fluorescence labeled peptide 5FAM-GGGPATPKKAKKL-COOH, substrate for CDK1/Cyclin B, CDK2/Cyclin A, CDK2/CyclinE, CDK3/CyclinE, CDK5/p25, CDK5/p35,CDK6/Cyclin D3,sufficient for 50,000 data points based on 100 nM in reaction	R7257
FAM-ERKTIDE 8K dp Fluorescence labeled peptide IPTTPITTTYFFFK-5FAM-COOH, substrate for Erk1, Erk2, p38 (α,β,γ,δ isoforms),sufficient for 8,000 data points based on 100 nM in reaction	R7292
TAMRA-EGFR-DERIVED PEPTIDE 8K dp Fluorescence labeled peptide LVEPLTPSGEAPNQK-5TAMRA-NH2, substrate for JNK1, JNK2, JNK3, p38 (α,β,γ,δ isoforms),sufficient for 8,000 data points based on 100 nM in reaction	R7307
FAM-CK1TIDE 8K dp Fluorescence labeled peptide 5FAM-HAAIGDDDDAYSITA-NH2, substrate for CK1, CK1δ,sufficient for 8,000 data points based on 100 nM in reaction	R7311
FAM-CK1TIDE 50K dp Fluorescence labeled peptide 5FAM-HAAIGDDDDAYSITA-NH2, substrate for CK1, CK1δ,sufficient for 50,000 data points based on 100 nM in reaction	R7312
TAMRA-PKATIDE 8K dp Fluorescence labeled peptide 5TAMRA-GRTGRRNSI-COOH, substrate for PKA, PKG,sufficient for 8,000 data points based on 100 nM in reaction	R7313
FAM-EGFR-DERIVED PHOSPHOPEPTIDE 8K dp Fluorescence labeled phosphopeptide LVEPL-pT-PSGEAPNQ(K-5FAM)-COOH, substrate for assay calibration,sufficient for 8,000 data points based on 100 nM in reaction	R7319
TAMRA-CDK7TIDE 8K dp Fluorescence labeled peptide 5TAMRA-YSPTSPSYSPTSPSYSPTSPS-COOH, substrate for CDK7,sufficient for 8,000 data points based on 100 nM in reaction	R7352
TAMRA-ROSTIDE 50K dp Fluorescence labeled peptide 5TAMRA-KKKSPGEYVNIEFG-NH2, substrate for Ros, Met,sufficient for 50,000 data points based on 100 nM in reaction	R7383
FAM-P38TIDE 8K dp Fluorescence labeled peptide 5FAM-IPTTPITTTYFFFK-NH2, substrate for Erk1, Erk2, p38 (α,β,γ,δ isoforms),sufficient for 8,000 data points based on 100 nM in reaction	R7434
FAM-CAM KIADR/SYNAPSIN CHIMERA PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-LKKLRRRSDANF-NH2, substrate for CaMKI,sufficient for 8,000 data points based on 100 nM in reaction	RP7005
FAM-PKCε PSEUDOSUBSTRATE DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-RKRQGSVRRRVH-OH, substrate for PKC,sufficient for 8,000 data points based on 100 nM in reaction	RP7030
FAM-PKCα PSEUDOSUBSTRATE DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-RFARKGSLRQKNV-COOH, substrate for PKCζ,sufficient for 8,000 data points based on 100 nM in reaction	RP7032
FAM-IP3R-DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-GRRESLTSFG-NH2, substrate for PKA, PKG,sufficient for 8,000 data points based on 100 nM in reaction	RP7035
FAM-ALTERNATE SYNTIDE2 8K dp Fluorescence labeled peptide 5FAM-PLSRTLSVSSLPGL-NH2, substrate for c-TAK,sufficient for 8,000 data points based on 100 nM in reaction	RP7045
FAM-CREBTIDE 8K dp Fluorescence labeled peptide 5FAM-GEILSRRPSYRK-NH2, substrate for MSK1,sufficient for 8,000 data points based on 100 nM in reaction	RP7046
FAM-CDK7TIDE 8K dp Fluorescence labeled peptide 5FAM-YSPTSPSYSPTSPSYSPTSPS-OH, substrate for TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction	RP7116
FAM-DYRKTIDE 8K dp Fluorescence labeled peptide 5FAM-EPPQSQEAFADLWK-NH2, substrate for TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction	RP7119
FAM-P53 DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-RRRFRPASPLRGPPK-OH, substrate for Dyrk1A, TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction	RP7120
FAM-MBP DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-ATGPLSPGPFGRR-OH, substrate for Erk2, TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction	RP7123
FAM-P44 DERIVED PEPTIDE (AA200-218) 8K dp Fluorescence labeled peptide 5FAM-FLTEYVATRWYRAPEIMLN-NH2, substrate for MAPK, TKL, STE, CK1, CMGC,sufficient for 8,000 data points based on 100 nM in reaction	RP7131
FAM-EXTENDED PLM DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-GTFRSSIRRLSTRRR-OH, substrate for Nek2, IRAK4,sufficient for 8,000 data points based on 100 nM in reaction	RP7140
FAM-RS DOMAIN DERIVED PEPTIDE 8K dp Fluorescence labeled peptide 5FAM-GRSRSRSRSR-OH, substrate for SPRK1, IRAK4,sufficient for 8,000 data points based on 100 nM in reaction	RP7153
FAM-PKCε PSEUDOSUBSTRATE DERIVED PEPTIDE 50K dp Fluorescence labeled peptide 5FAM-ERMRPRKRQGSVRRRV-NH2, substrate for PKCγ, Pim1,sufficient for 50,000 data points based on 100 nM in reaction	RP7548
FAM-GENERIC SER/THR PROTEIN KINASES SUBSTRATE PEPTIDE 50K dp Fluorescence labeled peptide 5FAM-KKRKSSLRRWSPLTPRQMSFDC-NH2, substrate for PKA, PKC, CAMK, MAPK, TKL, STE, CK1, CMGC,sufficient for 50,000 data points based on 100 nM in reaction	RP7609
FAM-CDK7TIDE 50K dp Fluorescence labeled peptide 5FAM-YSPTSPSYSPTSPSYSPTSPS-OH, substrate for TKL, STE, CK1, CMGC,sufficient for 50,000 data points based on 100 nM in reaction
FAM-EXTENDED PLM DERIVED PEPTIDE 50K dp Fluorescence labeled peptide 5FAM-GTFRSSIRRLSTRRR-OH, substrate for Nek2, IRAK4,sufficient for 50,000 data points based on 100 nM in reaction	RP7640

Additional discounted configurations available for customers performing several high-throughput screens in a year. Please contact your local sales representative or call 1-800-635-5577 for more information.

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