XP Media and CloneMedia Complete Kit for Mouse Hybridoma Generation

XP Media and CloneMedia Complete Kit for Mouse Hybridoma Generation

  • $954.00

The XP Media and CloneMedia for Mouse Hybridoma Generation is a complete solution that supports all stages of hybridoma cell line development from fusion to scale up. Optimized to support the selection and growth of hybridoma clones using the Molecular Devices ClonePix systems, the kit is also compatible with other appropriate methods. 

Key Advantages:
  • The semi-solid CloneMedia method of cloning prevents the overgrowth of fast-growing clones, allowing for the selection of potentially valuable slow-growing clones
  • Hands-on time can be reduced when the workflow is combined with a ClonePix system. Hypoxanthine-aminopterin-thymidine (HAT) selection and cloning of hybridomas are accomplished in one step, minimizing the required time and materials

Our XP Media and CloneMedia Complete Kit for Mouse Hybridoma Generation (P/N K8861) is composed of the following components. All components can be sold separately.

Component Description
 XP Media Pre-Fusion Myeloma Growth Medium and Hybridoma Expansion Medium (without HT)
P/N K8862
Supports the growth of myeloma cells before fusion. Also supports expansion of hybridoma clones. Does not contain hypoxanthine or thymidine (HT).
XP Media Hybridoma Fusion Medium
P/N K8863
Used to wash cells before fusion and during fusion process. Does not contain supplements to support growth.
XP Media Hybridoma Fusion Recovery Medium
P/N K8864
Used to promote hybridoma viability after the fusion process but before clone selection. Does not contain hypoxanthine, aminopterin, and thymidine (HAT).
CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (with HAT)
P/N K8865
Used after fusion of splenocytes and myeloma cells to select and clone hybridomas in one step. Methycellulose-based formula optimized for colony formation. Equally suitable for fresh fusions and for stable hybridoma cell lines.
XP Media Hybridoma Growth Medium (with HT)
P/N K8866
Optimized for hybridoma expansion following clone selection and colony picking. Contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin from the selection process.
Hybridoma Polyethylene Glycol (PEG) for Cell Fusion
P/N K8868
Used for the fusion of mouse splenocytes and parental myeloma cells to generate hybridomas. PEG is present as a 50% solution in DMEM.

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