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Secondary Antibodies

Secondary Antibodies

  • $129.00




    Compatible with the ScanLater™ Western Blot Detection Cartridge, these secondary antibodies enable the first western blot detection capability in a microplate reader. Utilizing this substrate-free assay, researchers can achieve equivalent sensitivity to traditional chemiluminescence and fluorescence-based assays, while consolidating western blot and ELISA applications onto a single reader.

     


     

    The ScanLater Western Blot secondary antibodies are conjugated with a Europium label to enable detection via time-resolved fluorescence (TRF).  TRF detection is optimal for quantitating as little as femtogram amounts of protein. Using this novel western blot method, researchers can:

    • Eliminate time-dependent substrate addition steps
    • Sustain blot signal stability for at least one month
    • Enhance assay sensitivity using TRF method to reduce background noise
    • Maintain femtogram to picogram protein sensitivity similar to traditional western blot detectors

    Features

    • Time-resolved fluorescence detection
    • Europium-labeled goat anti-rabbit, goat anti-mouse, donkey anti-rat, donkey anti-goat or streptavidin labeled antibodies
    • Compatible with nitrocellulose and PVDF membranes
    • 340/80 nm excitation with 616/10 nm emission range
    • Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3 and Paradigm®Multi-Mode Platforms

     

    Available Configurations:

    Product Name Volume Part Number
    ScanLater 5x Blocking Buffer 120 mL R8207
    ScanLater Wash Buffer 100 mL R8206
    ScanLater Goat Anti-Mouse Eu-labeled Antibody 60 μG R8208
    ScanLater Goat Anti-Rabbit Eu-labeled Antibody 60 μG R8209
    ScanLater Eu-labeled Streptavidin 60 μG R8212
    Europium-labeled Donkey Anti-Rat Antibody 60 
    μG
    R8229

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